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tcr γδ  (fluidigm)


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    fluidigm tcr γδ
    a , sjTREC quantification in HDs (black dots), heterozygous donors (gray dots) and individuals with CBL -LOH (colored dots), as determined by qPCR of whole-blood DNA; WBCs, white blood cells. b , Recent thymic emigrant CD4 + and CD8 + T cells quantified in peripheral fresh blood by mass cytometry and gating of CD31 + cells among naive T cells; data are shown as mean ± s.d. The statistical significance of differences was assessed by multiple two-sided Mann–Whitney tests, with correction for multiple testing; * P < 0.05 and ** P < 0.005. c , Quantification of the indicated T cell subsets in the peripheral blood of HDs, heterozygous HDs and individuals with CBL -LOH of the indicated ages as determined by mass cytometry; data are shown as mean ± s.d. The statistical significance of differences was assessed by multiple two-sided Mann–Whitney tests, with correction for multiple testing; * P < 0.05 and ** P < 0.005. In b and c , controls 0–3 years old ( n = 2), controls 4–15 years old ( n = 9), controls 16–100 years old ( n = 28), pediatric participants (LOH) ( n = 5), adult participants (LOH) ( n = 2) and heterozygous individuals ( n = 3). d , Percentage of dead cells in cultures of activated fresh PBMCs from HDs, the heterozygous father and participants (P1–P3) after 5 days of <t>TCR</t> stimulation, as determined by dead cell marker staining and flow cytometry; n = 3 HDs and n = 3 patient; data are shown as mean ± s.d. e , f , Cell division index of CD4 + ( e ) and CD8 + ( f ) T cells of HDs, the heterozygous father and participants (P1–P3) after 5 days of the indicated TCR stimulation, as determined by dilution of CFSE; n = 3 HDs and n = 3 patients; data are shown as mean ± s.d. g , Cytokine response by STAT5 phosphorylation (left) and cytokine production (right) by T cell blasts that are homozygous (red), heterozygous (gray) and homozygous WT for CBL Ub LOF variants following the indicated stimuli. The bars show the mean of the displayed data points (one for each T blast line); NS, not significant; TEMRA, terminally differentiated effector memory T cells; MAIT, mucosal-associated invariant T cells; MFI, median fluorescence intensity; PHA, phytohemagglutinin; PMA, phorbol 12-myristate 13-acetate.
    Tcr γδ, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tcrgd+152sm/Anti-Human+TCRgd+(11F2)-152Sm/pmc12864045-14-2-8
    Average 93 stars, based on 18 article reviews
    tcr γδ - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and function"

    Article Title: Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and function

    Journal: Nature Immunology

    doi: 10.1038/s41590-025-02381-7

    a , sjTREC quantification in HDs (black dots), heterozygous donors (gray dots) and individuals with CBL -LOH (colored dots), as determined by qPCR of whole-blood DNA; WBCs, white blood cells. b , Recent thymic emigrant CD4 + and CD8 + T cells quantified in peripheral fresh blood by mass cytometry and gating of CD31 + cells among naive T cells; data are shown as mean ± s.d. The statistical significance of differences was assessed by multiple two-sided Mann–Whitney tests, with correction for multiple testing; * P < 0.05 and ** P < 0.005. c , Quantification of the indicated T cell subsets in the peripheral blood of HDs, heterozygous HDs and individuals with CBL -LOH of the indicated ages as determined by mass cytometry; data are shown as mean ± s.d. The statistical significance of differences was assessed by multiple two-sided Mann–Whitney tests, with correction for multiple testing; * P < 0.05 and ** P < 0.005. In b and c , controls 0–3 years old ( n = 2), controls 4–15 years old ( n = 9), controls 16–100 years old ( n = 28), pediatric participants (LOH) ( n = 5), adult participants (LOH) ( n = 2) and heterozygous individuals ( n = 3). d , Percentage of dead cells in cultures of activated fresh PBMCs from HDs, the heterozygous father and participants (P1–P3) after 5 days of TCR stimulation, as determined by dead cell marker staining and flow cytometry; n = 3 HDs and n = 3 patient; data are shown as mean ± s.d. e , f , Cell division index of CD4 + ( e ) and CD8 + ( f ) T cells of HDs, the heterozygous father and participants (P1–P3) after 5 days of the indicated TCR stimulation, as determined by dilution of CFSE; n = 3 HDs and n = 3 patients; data are shown as mean ± s.d. g , Cytokine response by STAT5 phosphorylation (left) and cytokine production (right) by T cell blasts that are homozygous (red), heterozygous (gray) and homozygous WT for CBL Ub LOF variants following the indicated stimuli. The bars show the mean of the displayed data points (one for each T blast line); NS, not significant; TEMRA, terminally differentiated effector memory T cells; MAIT, mucosal-associated invariant T cells; MFI, median fluorescence intensity; PHA, phytohemagglutinin; PMA, phorbol 12-myristate 13-acetate.
    Figure Legend Snippet: a , sjTREC quantification in HDs (black dots), heterozygous donors (gray dots) and individuals with CBL -LOH (colored dots), as determined by qPCR of whole-blood DNA; WBCs, white blood cells. b , Recent thymic emigrant CD4 + and CD8 + T cells quantified in peripheral fresh blood by mass cytometry and gating of CD31 + cells among naive T cells; data are shown as mean ± s.d. The statistical significance of differences was assessed by multiple two-sided Mann–Whitney tests, with correction for multiple testing; * P < 0.05 and ** P < 0.005. c , Quantification of the indicated T cell subsets in the peripheral blood of HDs, heterozygous HDs and individuals with CBL -LOH of the indicated ages as determined by mass cytometry; data are shown as mean ± s.d. The statistical significance of differences was assessed by multiple two-sided Mann–Whitney tests, with correction for multiple testing; * P < 0.05 and ** P < 0.005. In b and c , controls 0–3 years old ( n = 2), controls 4–15 years old ( n = 9), controls 16–100 years old ( n = 28), pediatric participants (LOH) ( n = 5), adult participants (LOH) ( n = 2) and heterozygous individuals ( n = 3). d , Percentage of dead cells in cultures of activated fresh PBMCs from HDs, the heterozygous father and participants (P1–P3) after 5 days of TCR stimulation, as determined by dead cell marker staining and flow cytometry; n = 3 HDs and n = 3 patient; data are shown as mean ± s.d. e , f , Cell division index of CD4 + ( e ) and CD8 + ( f ) T cells of HDs, the heterozygous father and participants (P1–P3) after 5 days of the indicated TCR stimulation, as determined by dilution of CFSE; n = 3 HDs and n = 3 patients; data are shown as mean ± s.d. g , Cytokine response by STAT5 phosphorylation (left) and cytokine production (right) by T cell blasts that are homozygous (red), heterozygous (gray) and homozygous WT for CBL Ub LOF variants following the indicated stimuli. The bars show the mean of the displayed data points (one for each T blast line); NS, not significant; TEMRA, terminally differentiated effector memory T cells; MAIT, mucosal-associated invariant T cells; MFI, median fluorescence intensity; PHA, phytohemagglutinin; PMA, phorbol 12-myristate 13-acetate.

    Techniques Used: Mass Cytometry, MANN-WHITNEY, Marker, Staining, Flow Cytometry, Phospho-proteomics, Fluorescence

    Related Articles

    other:

    Article Title: Subsets of ILC3-ILC1-like cells generate a diversity spectrum of ILCs in human mucosal tissues.
    Article Snippet: All the following antibodies were purchased from Fluidigm and included in the panel CD45–089Y (3089003B); CD196–141Pr (3141003A); CD19–142Nd (3142001B); CD117–143Nd (3143001B); CD4–145Nd (3145001B); CD8a-146Nd (3146001B); CD25–149Sm (3149010B); FceR-150Nd (3150027B); CD138–150Nd (3150012B); CD103–151Eu (3151011B); TCRgd-152Sm (3152008B); TIGIT-153Eu (3153019B); CD3–154Sm (3154003B); CD85j-156Gd (3156020B); CD194–158Gd (3158032A); CD161–159Tb (3159004B); CD39–160Gd (3160004B); CD27–162Dy (3162009B); CD45RO-165Ho (3165011B); CD34–166Er (3166012B); CD127–168Er (3168017B); CD159–169Tm (3169013B); CD45RA-170Er(3170010B); CD226–171Yb (3171013B); CD354(TREM1)-172Yb (3172022B); CD94–174Yb (3174015B); CD14–175Lu (3175015B); CD56–176Yb (3176009B) and CD16–299Bi (3209002B).



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    Image Search Results


    a , sjTREC quantification in HDs (black dots), heterozygous donors (gray dots) and individuals with CBL -LOH (colored dots), as determined by qPCR of whole-blood DNA; WBCs, white blood cells. b , Recent thymic emigrant CD4 + and CD8 + T cells quantified in peripheral fresh blood by mass cytometry and gating of CD31 + cells among naive T cells; data are shown as mean ± s.d. The statistical significance of differences was assessed by multiple two-sided Mann–Whitney tests, with correction for multiple testing; * P < 0.05 and ** P < 0.005. c , Quantification of the indicated T cell subsets in the peripheral blood of HDs, heterozygous HDs and individuals with CBL -LOH of the indicated ages as determined by mass cytometry; data are shown as mean ± s.d. The statistical significance of differences was assessed by multiple two-sided Mann–Whitney tests, with correction for multiple testing; * P < 0.05 and ** P < 0.005. In b and c , controls 0–3 years old ( n = 2), controls 4–15 years old ( n = 9), controls 16–100 years old ( n = 28), pediatric participants (LOH) ( n = 5), adult participants (LOH) ( n = 2) and heterozygous individuals ( n = 3). d , Percentage of dead cells in cultures of activated fresh PBMCs from HDs, the heterozygous father and participants (P1–P3) after 5 days of TCR stimulation, as determined by dead cell marker staining and flow cytometry; n = 3 HDs and n = 3 patient; data are shown as mean ± s.d. e , f , Cell division index of CD4 + ( e ) and CD8 + ( f ) T cells of HDs, the heterozygous father and participants (P1–P3) after 5 days of the indicated TCR stimulation, as determined by dilution of CFSE; n = 3 HDs and n = 3 patients; data are shown as mean ± s.d. g , Cytokine response by STAT5 phosphorylation (left) and cytokine production (right) by T cell blasts that are homozygous (red), heterozygous (gray) and homozygous WT for CBL Ub LOF variants following the indicated stimuli. The bars show the mean of the displayed data points (one for each T blast line); NS, not significant; TEMRA, terminally differentiated effector memory T cells; MAIT, mucosal-associated invariant T cells; MFI, median fluorescence intensity; PHA, phytohemagglutinin; PMA, phorbol 12-myristate 13-acetate.

    Journal: Nature Immunology

    Article Title: Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and function

    doi: 10.1038/s41590-025-02381-7

    Figure Lengend Snippet: a , sjTREC quantification in HDs (black dots), heterozygous donors (gray dots) and individuals with CBL -LOH (colored dots), as determined by qPCR of whole-blood DNA; WBCs, white blood cells. b , Recent thymic emigrant CD4 + and CD8 + T cells quantified in peripheral fresh blood by mass cytometry and gating of CD31 + cells among naive T cells; data are shown as mean ± s.d. The statistical significance of differences was assessed by multiple two-sided Mann–Whitney tests, with correction for multiple testing; * P < 0.05 and ** P < 0.005. c , Quantification of the indicated T cell subsets in the peripheral blood of HDs, heterozygous HDs and individuals with CBL -LOH of the indicated ages as determined by mass cytometry; data are shown as mean ± s.d. The statistical significance of differences was assessed by multiple two-sided Mann–Whitney tests, with correction for multiple testing; * P < 0.05 and ** P < 0.005. In b and c , controls 0–3 years old ( n = 2), controls 4–15 years old ( n = 9), controls 16–100 years old ( n = 28), pediatric participants (LOH) ( n = 5), adult participants (LOH) ( n = 2) and heterozygous individuals ( n = 3). d , Percentage of dead cells in cultures of activated fresh PBMCs from HDs, the heterozygous father and participants (P1–P3) after 5 days of TCR stimulation, as determined by dead cell marker staining and flow cytometry; n = 3 HDs and n = 3 patient; data are shown as mean ± s.d. e , f , Cell division index of CD4 + ( e ) and CD8 + ( f ) T cells of HDs, the heterozygous father and participants (P1–P3) after 5 days of the indicated TCR stimulation, as determined by dilution of CFSE; n = 3 HDs and n = 3 patients; data are shown as mean ± s.d. g , Cytokine response by STAT5 phosphorylation (left) and cytokine production (right) by T cell blasts that are homozygous (red), heterozygous (gray) and homozygous WT for CBL Ub LOF variants following the indicated stimuli. The bars show the mean of the displayed data points (one for each T blast line); NS, not significant; TEMRA, terminally differentiated effector memory T cells; MAIT, mucosal-associated invariant T cells; MFI, median fluorescence intensity; PHA, phytohemagglutinin; PMA, phorbol 12-myristate 13-acetate.

    Article Snippet: 152Sm , TCR-γδ , 11F2 , 3152008B , Fluidigm.

    Techniques: Mass Cytometry, MANN-WHITNEY, Marker, Staining, Flow Cytometry, Phospho-proteomics, Fluorescence

    Journal: Cell reports

    Article Title: Multimodal analysis of dysregulated heme metabolism, hypoxic signaling, and stress erythropoiesis in Down syndrome

    doi: 10.1016/j.celrep.2024.114599

    Figure Lengend Snippet:

    Article Snippet: Mouse monoclonal anti-human TCRgd (Clone 11F2) , Fluidigm , Cat # 3152008B, RRID: AB_2687643.

    Techniques: Binding Assay, Isolation, Biomarker Discovery, Staining, Antibody Labeling, Clinical Proteomics, Mass Cytometry, Software, Sequencing

    Journal: iScience

    Article Title: Defects in NK cell immunity of pediatric cancer patients revealed by deep immune profiling

    doi: 10.1016/j.isci.2024.110837

    Figure Lengend Snippet:

    Article Snippet: Anti-TCR-γδ (clone 11F2, conjugated to 152Sm) , Standard BioTools , Cat#3152008B.

    Techniques: Purification, Clinical Proteomics, Recombinant, Blocking Assay, Staining, Saline, Mass Cytometry, Software, Cytometry